Neurogliaform cell type

Using genetic fate-mapping in combination with single-cell transcriptomics, we isolated and sequenced upper-layers (ULs) cortical NGCs. By aligning their transcriptomes to a reference atlas (Tasic et al., 2018), we revealed that ULs NGCs are molecularly different compared to other Htr3a-expressing interneurons (Non-NGCs).

Notably, these two cell type families express a combinatorial code of transcription factors (TFs). Specifically, while NGCs show an enriched expression of the TOX family of TFs (Tox2, Tox3), non-NGCs specifically express the NPAS family (Npas2, Npas3).

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DEGs Volcano plot

Cell type gene architectures.
Expression fold-change (x-axis) vs. celltype model weights (y-axis).

Postnatal landscape (Tox2)

Maturation model weights (x-axis) vs. celltype model weights (y-axis).

Postnatal waves (Tox2)

Gene smoothed expression across time.
Maturation model weights (x-axis) vs. logRPM RNA (y-axis).